Extended Data Fig. 9: Composition of PDIA6 chaperone condensates.
From: A multichaperone condensate enhances protein folding in the endoplasmic reticulum
a, Co-localization of overexpressed PDIA6–GFP (pink) and endogenous Grp94 (blue) (top), overexpressed PDIA6–GFP (pink) and endogenous ERdj3 (blue) (bottom) and overexpressed PDIA6–GFP (pink) and endogenous PDIA1 (blue) (right) in fixed HeLa cells. PDIA6 was visualized using the eGFP signal. Scale bar, 10 μm, insert 5 μm. b, Co-localization of overexpressed PDIA6–GFP (pink) and endogenous calnexin (blue) in fixed HeLa cells. PDIA6 was visualized using the eGFP signal. Scale bar, 10 μm, insert 5 μm. Fluorescence intensities across the condensates were measured along the yellow line and are shown as histograms (right). Representative quantification from 9 cells from three independent replicates. c, Co-localization of endogenous BiP (pink) and endogenous calreticulin (blue) (top) or calnexin (blue) (bottom) in fixed HeLa cells. Scale bar, 10 μm, insert 5 μm. Fluorescence intensities across the condensates were measured along the yellow line and are shown as histograms (right).
