Extended Data Fig. 4: PDIA6 methyl group and amide backbone NMR assignments.
From: A multichaperone condensate enhances protein folding in the endoplasmic reticulum
a, 2D [13C,1H]-HMQC spectrum of methyl-labelled PDIA6. Assignment of Met[13C1H]ε, Val-[13C1H]γ2 and Leu-[13C1H]δ2 methyl groups are indicated. Peaks annotated with a “t” correspond to residues in the C-terminal tail which could not be unambiguously assigned. b, Assigned methyl groups (pink spheres) displayed on the structure of PDIA6 established in Fig. 2. Blue spheres represent partially assigned residues in the C-terminal tail. c–e, 2D [15N,1H]-HSQC spectra of PDIA6 domain a0 (c), domain a (d) and domain ab (e). Sequence-specific resonance assignments are indicated. f, Percentages of assigned residues of methyl and amide groups in PDIA6 FL and domain constructs.
