Extended Data Fig. 4: TSS switching alters regulatory 5´UTR features and shapes the hypoxia-induced translatome in H9 cells.
From: Epigenetic alterations facilitate transcriptional and translational programs in hypoxia
a, Network plot displaying the results of Model 1. Percentages of explained and unexplained variance in translation efficiency, and contributions from each input category, are indicated. Connections between features (nodes) indicate substantial correlations. Node colours indicate the mode of translational regulation under hypoxia the feature is associated with. b, As in a, but modelling translational offsetting induced by hypoxia in H9 cells. c, Selection of the full network plot for Model 2, displaying the additional impact of adding TSS-switching signatures to Model 1 in explaining changes in translation efficiency under hypoxia in H9 cells. d, As in c, but modelling translational offsetting induced by hypoxia in H9 cells. e, Scatterplot comparing the change in TOPscore vs. the change in translation efficiency that occurs under hypoxia. Pearson’s correlation coefficient and P values from two-sided tests. r = -0.181, P = 2.53e-03. f, Scatterplot comparing 5´UTR lengthening events vs. translational offsetting that occurs under hypoxia. Pearson’s correlation coefficient and p values from two-sided tests indicated. r = -0.136, P = 4.61e-04. g, Bar plot indicating the number of transcripts that gain or lose 5´UTR elements identified in either Model 1 or 2 in H9 cells. Transcripts were considered if more than 10% of the expressed pool of 5´UTR isoforms gained or lost the 5´UTR element. The translation mode associated with each element is indicated by coloured squares below. Source numerical data are available in.
