Extended Data Fig. 5: TSS switching alters regulatory 5´UTR features and shapes the hypoxia-induced translatome in H9 cells.
From: Epigenetic alterations facilitate transcriptional and translational programs in hypoxia
a, Scatterplot comparing the composition of each 5´UTR feature identified in Model 1 versus the change in translation efficiency that occurs under hypoxia. Pearson’s correlation coefficient and P values from two-sided tests indicated. %GC r = -0.359, P = 9.37e-13; UAAAAA r = 0.271, P = 1.15e-07; CCACGCA r = 0.113, P = 2.97e-02; CCCUGC r = -0.178, P = 5.58e-04; CGCCCCG r = -0.152, P = 3.32e-03; CGGCGCC r = -0.137, P = 8.02e-03; GGAAGAAG r = 0.166, P = 1.29e-03; GAGGAAC r = 0.180, P = 4.85e-04; GAGACGA r = 0.181, P = 4.5e-04; CCCUCGA r = 0.148, P = 4.23e-03. b, Same as a for translational offsetting. %GC r = 0.36, P = 1.25e-27; AAGAAA r = -0.268, P = 2.01e-12; AAAAAA r = -0.208, P = 7.17e-10; AUUUUG r = -0.19, P = 2.01e-08; CUUCAC r = -0.145, P = 2.11e-05; UUAGAG r = -0.165, P = 1.19e-06; CCCAGGA r = -0.113, P = 9.47e-04; UGACGGA r = -0.074, P = 3.08e-02; GGUCCCCA r = -0.106, P = 1.9e-03; CCGCCGG r = -0.089, P = 9.09e-03. c, Network plot displaying the complete results of Model 2 in H9 cells. Percentages of explained and unexplained variance in translation efficiency, and contributions from each input category, are indicated. Connections between features (nodes) indicate substantial correlations. Node colours indicate the mode of translational regulation under hypoxia the feature is associated with. d, As in c, but modelling changes in translational offsetting induced by hypoxia in H9 cells. Source numerical data are available in.
