Extended Data Fig. 6: Hypoxia-induced TSS switching is associated with altered H3K4me3 and changes in nucleosome context.
From: Epigenetic alterations facilitate transcriptional and translational programs in hypoxia
a,b, PCA of quantification of H3K4me3 peaks surrounding TSSs from T47D (a) and H9 (b) cells grown in hypoxia and normoxia and quantified by ChIP-seq (n = 3 independent experiments). c, PCA of quantification of ATAC-seq peaks at TSSs from T47D cells grown in hypoxia and normoxia (n = 3 independent experiments). d, Immunoblots of H3K4me3, and H3, with α-tubulin as a loading control. C48 was titrated from 0 to 10 μM in T47D cells. Lysates were harvested after 24 h treatment (n = 3 independent experiments). e, PCA of quantification of H3K4me3 peaks surrounding TSSs from T47D cells treated with C48 or DMSO for 24 h quantified by ChIP-seq (n = 4 independent experiments). Unprocessed blots are available in.
