Extended Data Fig. 6: RNA‑FISH quantification and enhancer reporter activity for EIE 14. | Nature Cell Biology

Extended Data Fig. 6: RNA‑FISH quantification and enhancer reporter activity for EIE 14.

From: Enhancer activation from transposable elements in extrachromosomal DNA

Extended Data Fig. 6: RNA‑FISH quantification and enhancer reporter activity for EIE 14.The alternative text for this image may have been generated using AI.

a. Quantification of RNA-FISH signal on a per cell basis from COLO320-DM cells labeling MYC exon 2 and EIE 14 (see Methods for quantification method). n = 712 cells across 2 biological replicates. b. As in (a) but for COLO320-HSR cells. n = 681 cells across 2 biological replicates. c. Fold change in luciferase signal driven by the L1PA2 (part 1), L1M4a1 (part2) and combined (EIE14) regions of EIE 14 for n = 3 biological replicates. P-value MYC promoter (ctrl) vs part 1 p = 0.0006; p-value MYC promoter (ctrl) vs part 2 p = 0.0015; value MYC promoter (ctrl) vs part 1+part2 (EIE14) p = 0.0001 (p-values obtained from two-tailed unpaired t-test). Error bars are stanford deviations from the mean. Source numerical data and images are available in source data.

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