Extended Data Fig. 7: Further characterization of embryoids at S7.
a, A representative bright field image of embryo-like structures at S7. Scale bar, 500 μm. Similar images were obtained in at least 3 independent experiments. b, Percentage of embryoids at S7 expressing T with correct spatial organization. Data are presented as mean ± SD, n = 48 for S7 embryoids. c, Percentage of embryoids at S7 expressing FOXA2 and SOX17 with correct spatial organization. Data are presented as mean ± SD, n = 67 for S7 embryoids. d, Dot plots showing the average levels and proportion of cells expressing cell type-specific marker genes in S7 embryoids. Cell type-specific marker genes for different lineages in E7.5 mouse embryos were identified using published single-cell transcriptomic datasets (E-MTAB-6967). Expressions of these marker genes in different linages from E7.5 mouse embryos are shown as the control. Em_VE, Embryonic visceral endoderm; RN, Rostral neurectoderm; PS, Primitive Streak; PE, Parietal endoderm; Par_meso, Paraxial mesoderm; HP, Haematoendothelial progenitors; Ex_meso, Extraembryonic mesoderm; Ex_VE, Extraembryonic visceral endoderm; Ex_ecto, Extraembryonic ectoderm; EPI, Epiblast; DE, Definitive endoderm; Caudal_meso, Caudal mesoderm. e, Feature plots showing the expression of PGC classical genes. f, Correlation of gene expression for each identified lineage between embryo-like structures at S7 and natural E7.5 embryos. Correlation coefficients and p values are indicated. p values were calculated using the two-sided t-test. g, Feature plots showing expression of indicated lineage markers in S7 embryoids (left) and natural E7.5 embryos (right). Bmp4, Eomes, Sox2, Cdx2 and Sox21 mark chorion progenitors; Irx4, Esx1 and Id1 mark chorion; Hand1, Ascl2 and Chsy1 mark EPC (ectoplacental cone cell); Adm, Mmp9 and Pecam1 mark TGC (Trophoblast Giant Cell) progenitors (E-MTAB-6967).
