Extended Data Fig. 10: Independent validation of the stepwise induction protocol by Guan laboratory.
a, Bright field images of totipotent-like cell-derived embryo-like structures at different stages. Scale bars, 100 μm. Similar images were obtained in at least 3 independent experiments. b, Quantification of embryoid formation efficiency from S1 to S8. Data are mean ± SD, n = 240 for S1 from 6 experiments, 240 for S2 from 6 experiments, 240 for S3 from 6 experiments, 200 for S4 from 5 experiments, 200 for S5 from 5 experiments, 47 for S6 from 4 experiments, 67 for S7 from 4 experiments, 90 for S8 from 3 experiments. c, Q-PCR analysis showing the effect of S1 on the expression levels of classical totipotent marker genes. Samples were treated for three days before their collection. n = 2 biological replicates. d, Immunofluorescent analysis showing the expression of CDX2 and OCT4 in blastoids at S4. n = 3 S4 blastoids from 2 experiments. Scale bar, 50 μm. e, Immunofluorescent analysis showing the expression of SOX17 and OCT4 in blastoids at S4. n = 3 S4 blastoids from 2 experiments. Scale bar, 50 μm. f, Immunofluorescent analysis showing the expression of CER1 at S5. n = 4 from 2 experiments. Scale bar, 50 μm. g, Immunofluorescent analysis showing the expression of OCT4 and SOX17 at S6. n = 3 from 2 experiments. Scale bar, 50 μm. h, Immunofluorescent analysis showing the expression of OCT4 and T at S7. n = 4 from 2 experiments. Scale bar, 50 μm. i, Immunofluorescent analysis showing the expression of SOX17 and FOXA2 at S7. n = 3 from 2 experiments. Scale bar, 50 μm.
