Extended Data Fig. 1: Proteome dynamics during young small intestinal epithelium regeneration.
From: Polyamines sustain epithelial regeneration in aged intestines by modulating protein homeostasis
a Quantification of crypt length in the indicated groups. PBS-treated mice from different days were combined. Each dot represents one mouse. n = 16 PBS, n = 5 day 2, n = 6 day 5, n = 8 day 7 5-FU. Data are presented as mean ± SD. p-value was calculated using two-tailed Welch’s t test. b Number of quantified protein groups in the indicated treatments and time points. PBS-treated young mice: n = 5 day 2, n = 5 day 5, n = 4 day 7. 5-FU-treated young mice: n = 5 day 2, n = 5 day 5, n = 4 day 7. c Schematic of experimental design, proteomics data analysis and visualization strategy. d Representative abundance profiles for proteins affected by 5-FU treatment and belonging to different clusters. Protein profiles are plotted using a loess smooth function. The shaded area around the regression line represents the 95% confidence interval. n = 14 PBS, n = 5 day 2, n = 5 day 5, n = 4 day 7 5-FU. e Protein abundance profiles of 40S (26 proteins) and 60S (41 proteins) ribosome complexes. Protein profiles are plotted using a loess smooth function. The shaded area around the regression line represents the 95% confidence interval. n = 14 PBS, n = 5 day 2, n = 5 day 5, n = 4 day 7 5-FU. f Protein abundance profiles of components of the MCM complex. Protein profiles are plotted using a loess smooth function. The shaded area around the regression line represents the 95% confidence interval. n = 14 PBS, n = 5 day 2, n = 5 day 5, n = 4 day 7 5-FU. g Left: Schematic of colony forming assay. Middle: Representative pictures of organoids in the different groups. Right: quantification of the growing organoids in the indicated groups normalized to the average of PBS, scale bar, 200 µm. n = 4 PBS; n = 3 day 2, n = 4 day 5, n = 4 day 7 5-FU. Data are presented as mean ± SD. p-value was calculated using two-tailed Welch’s t-test.
