Extended Data Fig. 3: H3K36me2 reprogramming in early embryos depends on zygotic transcription.
From: Reprogramming of H3K36me2 guides lineage-specific post-implantation de novo DNA methylation
a, Left, schematic showing treatment of PN5 zygotes with α-amanitin (α-Ama) for 24 h. Right, heatmaps showing the RNA levels of oocyte-specific, major ZGA, HK, and inactive genes, and H3K36me2/3 near these genes ( ± 1 × gene body) upon treatment with α-amanitin. b, The UCSC genome browser views comparing the H3K36me2/3 in control and α-amanitin-treated late 2C embryos. Active gene bodies are shaded. c, Left, schematic showing treatment of PN5 zygotes with aphidicolin to block cell cycle. Right, heatmaps showing the RNA levels of oocyte-specific, major ZGA, HK, and inactive genes, and H3K36me2/3 near these genes ( ± 1 × gene body) upon treatment with aphidicolin for 44-46 h. d, The UCSC genome browser views comparing the H3K36me2/3 in control (8C) and aphidicolin-treated (2C) embryos. e, Bar chart showing the expression of H3K36me2 and H3K36me3 regulators in control (8C) and α-amanitin-treated (2C) embryos (n = 2 biological replicates). Source numerical data are available in source data.
