Extended Data Fig. 1: Dynamic H3K36me2 in mouse oocytes and early embryos.
From: Reprogramming of H3K36me2 guides lineage-specific post-implantation de novo DNA methylation
a, Immunostaining showing H3K36me2 dynamics in mouse oocytes and early embryos. Dashed circles, chromatin in MII oocytes, and the maternal (♀) and paternal (♂) pronuclei in 1 C embryos (PN5 stage). NSN, non-surrounded nucleolus; SN, surrounded nucleolus; FGO, full-grown oocyte; MII, metaphase II; 1 C, 1-cell; 2 C, late 2-cell; 4 C, 4-cell; 8 C, 8-cell; Bl, blastocyst. PB, polar body. Scale bar, 50 μm. H3K36me2 is absent in sperm (arrows in ‘1 C’ panel). b, Jitter plot with mean ± SEM showing the relative H3K36me2 signals at each stage. M, maternal pronuclei; P, paternal pronuclei. The numbers of oocytes or embryos examined are 4 (NSN FGO), 8 (SN FGO), 8 (MII), 11 (PN5), 13 (2 C), 11 (4 C), 10 (8 C), and 10 (Bl). c, Box plots showing H3K36me2 (left) and H3K36me3 (right) in gene bodies of NSN FGOs, SN FGOs, and MII oocytes. Genes are ranked by their transcription levels in NSN FGOs. Center line, median; box, 25th and 75th percentiles; whiskers, 1.5 × IQR. d, Scatter plots comparing H3K36me2 and H3K36me3 (100-kb bin) in mouse NSN FGOs, SN FGOs, MII oocytes, and mESCs. The Pearson correlation coefficients are shown. Transcription (Trx) is active (+) in NSN FGOs and mESCs, inactive (-) in SN FGOs and MII oocytes. e, The UCSC genome browser views showing maternal (M) and paternal (P) H3K36me2 in mouse gametes and early embryos. Prospermatogonia (PSG) data are from a previous study17. f, Hierarchical clustering of mouse gametes and early embryos based on allelic H3K36me2 (5-kb bin). g, Box plot showing the differences of allelic H3K36me2 (5-kb bin) (paternal-maternal, P-M) normalized by total H3K36me2 (P + M) across the genome. Center line, median; box, 25th and 75th percentiles; whiskers, 1.5 × IQR. Source numerical data are available in source data.
