Extended Data Fig. 3: The PCH void in −rDNA embryos does not stain for DAPI, Fib, Mod, H3K9me2 or Propidium Iodide (RNA). | Nature Cell Biology

Extended Data Fig. 3: The PCH void in −rDNA embryos does not stain for DAPI, Fib, Mod, H3K9me2 or Propidium Iodide (RNA).

From: Hierarchical interactions between nucleolar and heterochromatin condensates are mediated by a dual-affinity protein

Extended Data Fig. 3

a, Representative images of fixed nuclei from late-stage +rDNA and −rDNA embryos stained for Fib (green) and DAPI (blue). Nuclei marked with white dashed box are enlarged and shown in the z plane. The yellow arrow points to the lack of Fib in the ‘PCH void’ in −rDNA nuclei. b, Representative images of fixed nuclei from +rDNA and -rDNA embryos stained for Mod (magenta) and DAPI (blue). The yellow arrow points to Mod localization in a +rDNA nucleus and dispersion in −rDNA. c, H3K9me2 (magenta), Fib (green) immunofluorescence and DAPI (blue) staining in +rDNA and −rDNA nuclei in late-stage Drosophila embryos. The yellow arrow points to the lack of H3K9me2 in the ‘PCH void’ in −rDNA nuclei. d, Representative images of fixed nuclei from late-stage +rDNA and −rDNA embryos stained with propidium iodide (green) and DAPI (blue) without (left) and with (right) RNase A. The yellow arrow points to the RNA staining in the nucleolus in +rDNA and lack of propidium iodide staining in the PCH void of −rDNA nuclei. e, Immunofluorescence images staining for Mxc, a marker for the Histone Locus body (magenta), Fib (green), and DAPI (grey) in +rDNA and −rDNA nuclei in early embryos (~cycle 15).

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