Extended Data Fig. 4: Characterization of P100 samples from cancer cell lines by cryo-EM and western blot.
From: Defining the reference proteomes for small extracellular vesicles and non-vesicular components
a, Representative cryo-EM images of crude sEV (P100) preparations from the indicated cancer cell lines, showing the presence of intact, vesicle-like structures of the expected size and morphology. Cryo-EM analysis was performed once for each cell line, yielding consistent results across n = 5 independent cancer cell lines. b, Western blot analysis of crude sEV (P100) preparations and corresponding whole-cell lysates for the indicated cell lines. The blots demonstrate the enrichment of positive sEV markers (CD9, Syntenin-1, CD63) and the depletion of intracellular contaminant markers (Calreticulin, GOLGA2/GM130) in the sEV fractions. Ponceau S staining is shown as loading control. Doted line represents different exposure time. The experiment was performed once for each cell line, demonstrating consistent marker enrichment profiles across n = 4 independent cancer cell lines.
