Extended Data Fig. 5: Inhibition of upstream glycolysis rescues aldolase loss-of-function phenotypes.
From: Fructose-1,6-bisphosphate couples glycolytic activity to cell adhesion
a, Inhibiting glycolysis lowers FA number and cell size to normal levels in aldolase knockdown cells. Representative confocal images of paxillin-labeled FAs in U-2 OS cells treated with indicated siRNAs followed by 48 h treatment with PBS or 25 mM 2-deoxy-D-glucose (2-DG). FA segmentation and cell outlines (red) are shown below. Corresponding quantification is shown in Fig. 3g, h. b, Inhibiting glycolysis rescues elevated FBP level in aldolase-depleted cells. Relative FBP levels measured in U-2 OS cells treated with control (-) or aldolase-specific siRNA (ALDOA, +) followed by 48 h treatment with PBS (-) or 25 mM 2-DG. Data were normalized to siCtrl + PBS and represent mean ± SEM; n = 4 independent experiments; two-sided One sample t-test (P values: (siALDOA) = 0.0227; (siCtrl+2DG) = 0.005; (siALDOA+2-DG) = 0.007). Ns, not significant; *p < 0.05, **p < 0.01. Scale bars, 25 µm.
