Fig. 4: FBP promotes FA assembly.
From: Fructose-1,6-bisphosphate couples glycolytic activity to cell adhesion
a,e, Reduced FBP levels associated with PFK depletion decrease FA assembly (a), whereas increased FBP levels upon aldolase knockdown increase the FA assembly rate (e). Representative TIRF microscopy time-lapse series of U-2 OS cells stably expressing eGFP–paxillin and treated with indicated siRNAs. Zoom-ins of the orange boxes are shown. b,c,f,g, A quantification of FA assembly rate (b,f) and disassembly rate (c,g). Data represent median and interquartile ranges. For b and c, n(siCtrl) = 34 and n(siPFK) = 39 cells from five independent experiments; for f and g, n(siCtrl) = 37 and n(siALDOA) = 35 cells from five independent experiments; two-sided Mann–Whitney test (P values (for b) = 0.0047; (for c) = 0.2941; (for f) = 0.0178; (for g) = 0.0492). d,h, A quantification of novel FAs formed over 4 h for siPFK (d) and siALDOA (h). Data represent median and interquartile ranges. For d, n(siCtrl) = 35 and n(siPFK) = 40 cells from five independent experiments; for h, n(siCtrl) = 37 and n(siALDOA) = 35 cells from five independent experiments; two-sided Mann-Whitney test (P values: (for d) <0.0001; (for h) = 0.0061). n.s., not significant; *P < 0.05, **P < 0.01, ****P < 0.0001. Scale bars, 25 µm and 10 µm for zoomed-in images.
