Extended Data Fig. 9: Transferrin and sphingomyelin co-localize in clathrin-coated pits.
From: Visualizing suborganellar lipid distribution using correlative light and electron microscopy
A: A representative image of a U2OS cell loaded with transferrin (purple) and SM(Y) (orange) and immuno co-stained for clathrin (blue). The basal PM is shown. Scale bar: 10 µm. ROIs show clathrin-coated pits and their co-localisation with SM(Y) and transferrin. Scale bar: 500 nm. B: Quantification of the mean fluorescence (FI) of SM or transferrin signal red out over the mask of clathrin-coated pits, normalized over the mean FI over the whole cell. Boxplots show the median (central line) and interquartile range (box bounds: Q1 to Q3), with Tukey-style whiskers extending to 1.5 × IQR. Individual data points are shown in grey. Three independent samples were measured (n(field of view): 18). Each field of view includes at least 5 cells. Source numerical data are available in source data.
