Extended Data Fig. 2: Non-photo-crosslinked bifunctional lipid probes are removed during automatic freeze substitution.
From: Visualizing suborganellar lipid distribution using correlative light and electron microscopy
A: U2OS cells were loaded for 4 min with PC(16:0 | Y), not photoactivated ( + Lipid -UV) or photoactivated ( + Lipid +UV), and prepared according to the “classic” lipid imaging protocol by chemical fixation, permeabilization, stain and imaging in PBS, or B: by embedding in HM20 and C: K11M according to the here proposed Lipid-CLEM workflow. The HM20 and K11M sections are 100 nm thin. Red-dotted lines show the cell outlines. Scale bars: 10 µm. The images of the right column are shown in Figs. 1c and 2a. The datasets for all conditions include 4 (A), 3 (B) and 3 (C) independent replicates, that yielded similar results.
