Extended Data Fig. 4: Effect of lipid loading on cellular signaling events.
From: Visualizing suborganellar lipid distribution using correlative light and electron microscopy
A: To test if lipid loading induces major signaling events, the localization of DAG and PA protein sensors, as well as intracellular Calcium levels, were monitored during lipid loading using live cell imaging. A drop of sensor intensity in the cytosol is expected for the PA and DAG sensor upon recruitment to other membranes, whereas the calcium sensor intensity increases upon binding Ca2+ in the cytosol. B: The traces of the three sensors are plotted as the sensor intensity in the cytosol normalized against the cytosolic sensor intensity at time point −40 s before adding the lipid. Images were acquired every 10 s. Representative images of the sensor intensity are shown for the timepoints −40 s, 10 s, 60 s, and 260 s, and for the Calcium sensor (C), PA sensor (D), and DAG sensor (E). Timepoint 0 marks the addition of the lipid. Scale bar: 10 µm. Three independent samples were measured (n: 30, per condition). Median and 95% confidence interval are plotted. Source numerical data are available in source data.
