Extended Data Fig. 10: SP110 and SP100 localization and complementation expression. | Nature Cell Biology

Extended Data Fig. 10: SP110 and SP100 localization and complementation expression.

From: An SP110–SP100 axis is a critical regulator of promyelocytic leukaemia body dynamics and mitotic fidelity

Extended Data Fig. 10: SP110 and SP100 localization and complementation expression.The alternative text for this image may have been generated using AI.

a. Western blot of stably expressed FLAG-SP110 variants in RPE1 SP110-KO background. b. Western blot of stably expressed FLAG-SP110 CARD variants RPE1 SP110-KO background. The indicated CARD replaces the SP110 CARD in the full-length SP110. c. Fluorescence microscopy imaging of stably expressed SP110-GFP (yellow) constructs in RPE1 WT cells treated with IFNβ. Scale bar, 20 µm. d. Western blot of stably expressed mCherry-HA-SP100 variants in RPE1 SP100-KO background. e. Fluorescence microscopy imaging of stably expressed mCherry-SP100 (yellow) constructs in RPE1 WT cells treated with IFNβ. Scale bar, 20 µm. f. Western blot of stably expressed mCherry-HA-SP100 CARD variants RPE1 SP100-KO background. The indicated CARD replaces the SP100 CARD in SP100A. All experiments were performed 2–3 independent times with similar results. Unprocessed blots are available in source data.

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