Extended Data Fig. 4: EGFR pathway activity is unaffected by HELDR, and EGFR does not affect HELDR expression.
From: An EGFR co-amplified lncRNA HELDR promotes glioblastoma malignancy through KAT7-driven gene programs
a, Diagram of EGFR, ELDR/HELDR gene locus and nearby genes in Chromosome (Chr) 7 genomic DNA71 or ecDNAs3,4 in GBM. b, Dot plot showing expression changes of the indicated genes at the EGFR and ELDR/HELDR loci in GSC17 cells after HELDR knockdown with two independent shRNAs. The box highlights SEC61G, the only gene significantly regulated by HELDR KD in both shRNAs. c, IB for EGFR and EGFR downstream signalling proteins in GSC34 or GSC11 cells with or without HELDR KD. d, e, IB of EGFR-pathway proteins (d) and qRT–PCR (n = 3) of HELDR (e) in GSCs with or without EGFR knockdown or treatment with the EGFR inhibitor erlotinib. β-actin is a sample processing control in c and d. All blots and qRT–PCR are representative of three independent experiments. Two-sided Wald test with Benjamini–Hochberg adjustment for multiple comparisons in b, one-way ANOVA with Dunnett’s post hoc test in e, Data are shown as mean ± s.d. (e).
