Extended Data Fig. 2: Loss of the HOIL-1 RBR impairs glycogen breakdown.
From: The atypical E3 ligase HOIL-1 safeguards the ribosome during cellular stress
a, Model system. b, Immunoblot analysis of AC16 cells. Arrowhead indicates ΔRBR HOIL-1. Asterisks indicate non-specific bands. c, Genomic DNA sequencing from AC16 cells confirming deletion and premature stop codon in HOIL-1ΔRBR cells. Red text is the gRNA target sequence. d, Fraction of cellular glycogen remaining after 1 h glucose starvation in AC16 cells. Pooled data from three independent experiments. Each data point is the mean of 3 biological replicates. Data are presented as mean ± s.d. e, Glucose-1-phosphate levels in AC16 cells after glucose starvation measured by LC-MS (n = 6 biological replicates). Data are presented as mean ± s.e.m. f, Immunoblot analysis of HOIL-1ΔRBR 786-O cells. g, Immunoblot analysis of HOIL-1ΔRING1 MEFs. P values were determined by unpaired two-tailed Student’s t-test (d) or two-way ANOVA followed by Dunnett’s post-hoc comparison (e). Source numerical data and uncropped blots are available in Source Data.
