Fig. 1: Quantifying the dynamic proteome from ESCs to gastruloids.
From: The proteomic landscape and temporal dynamics of human and mouse gastruloid development
a, Representative brightfield images of human RA-gastruloids and mouse gastruloids imaged over the course of their development. Scale bars, 10 μm. The experiments were independently reproduced five times with similar results. b, Multi-omics profiling workflow. We sampled two human cell lines (H9 and RUES2-GLR) and one mouse cell line (E14) at the indicated stages. c–e, Representative heatmaps depicting the temporal dynamics of RNAs (c), proteins (d) or phosphosites (e) for selected developmental marker transcripts, proteins or PTMs, respectively, across replicates and stages for both human and mouse. The colour scale for RNAs indicates log2(fold change) relative to the row mean. The colour scale for protein and phosphorylation data indicates scaled TMTpro reporter ion abundance. FC, fold change.
