Extended Data Fig. 4: Temporal protein profiling recapitulates systematic downregulation of OxPhos over mouse gastruloid differentiation.
From: The proteomic landscape and temporal dynamics of human and mouse gastruloid development
(a) Volcano plots depicting the DAPs between early (blue) and late (red) mouse gastruloids, where x-axis represents the log2 fold change between the two timepoints and y-axis represents the negative log10 of the BH-adjusted p-value (correcting for multiple hypothesis testing). Significance determined using the two-sided standard t-test. Labels indicate OxPhos subunits. (b) Dot plots indicating the GO terms enriched in DAPs between early and late mouse gastruloids. Color scales for dot plots indicate the BH-adjusted p-value and sizes of dots indicate the number of genes detected within each term. Significance determined using a one-sided hypergeometric test. (c) Schematic of OxPhos complexes. (d) Number of significantly changing OxPhos proteins in this study and Stelloo et al.42. (e) Comparison of the temporal dynamics of OxPhos proteins in mouse gastruloid development between this study (left) and Stelloo et al.42 (right); n = 3 biological replicates per timepoint. Significance determined using one-way ANOVA.
