Extended Data Fig. 7: Protein entry onto the LD surface through ER-LD contact sites. | Nature Cell Biology

Extended Data Fig. 7: Protein entry onto the LD surface through ER-LD contact sites.

From: Membrane bridges and nanodomain partitioning govern membrane protein targeting to lipid droplets

Extended Data Fig. 7: Protein entry onto the LD surface through ER-LD contact sites.The alternative text for this image may have been generated using AI.

a. Single-molecule tracks of SBP-LiveDrop 8 minutes after biotin release. Cells were treated with 250 µM oleic acid for 3 hours prior to release and labeled with 80 pM JFX554 for HILO imaging. Tracks transition from free motion (blue) to confined motion (orange) as proteins move from the ER membrane to the LD surface. All recorded tracks are shown in light grey. LDs are labeled with BODIPY 493/503 (green). Scale bar: 10 µm. b. Representative ER-to-LD trajectories, colored by motion state (blue: free, orange: confined); track start points are marked with stars. Scale bar: 500 nm. c. Overlay of coordinates where tracks switch from free to confined motion (from panel b) plotted over the LD channel (green).

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