Extended Data Fig. 4: Specific amino acid sequences in the hairpin region mediate nanodomain interaction.
From: Membrane bridges and nanodomain partitioning govern membrane protein targeting to lipid droplets
a. Cumulative density function probabilities of total nanodomain area per LD explored by WT (orange) and 3 W (blue). WTmedian = 0.018 µm2, 3 Wmedian = 0.018 µm2. P = 0.01. b. Normalized nanodomain engagement episodes were plotted as CDF for LD (left) and ER (right) localized trajectories of WT (orange) and 3 W (blue). Normalization was done by dividing number of episodes to track length. Median values for WTLD = 95, WTER = 85, 3 WLD = 140, 3 WER = 90. A two-sided Mann–Whitney test was used to assess the statistical significance of the difference in total nanodomain areas between WT and 3 W mutant. P = 0.02 (LD), P = 0.01 (ER). c. KDE-based density enrichment within nanodomains was quantified for WT (blue), 3 W (magenta) and 3W + KRR (orange). Enrichment was defined as the ratio of the mean KDE value at each dense region to the mean KDE of the full grid. Data were plotted as box plots for ER and LD membranes. d. Time-resolved density enrichment was plotted for ER-localized (purple) and LD-localized (orange) trajectory segments of WT (left), 3W (middle), and 3W + KRR (right). Segment lengths were varied to assess temporal trends, and enrichment was calculated as described in (a) for each time window. Density enrichment was quantified by calculating the ratio of the number of localizations per unit area in dense regions to that in the total KDE area. wild-type (WT) and mutant hairpins. Shaded area represents 95% confidence interval. Source numerical data are available in source data.
