Fig. 1: Cryo-ET of ERES in HaloTag-Sec23A RPE-1 cells.
From: In situ cryo-ET defines the ultrastructure of ER exit sites in human cells
a, Slice through the x–y plane of a reconstructed tomogram from a high-defocus, low-magnification tilt series from a FIB/SEM lamella of HaloTag-Sec23A RPE-1 cells, providing a panoramic view of cellular features. The ER is studded with ribosomes (pale blue arrowheads), while Golgi cisternae appear fenestrated and enriched with coated vesicles (purple arrowheads). b, Slice through the x–y plane of a reconstructed tomogram from a high-magnification tilt series collected in the area highlighted in the cyan box in a, acquired at conditions where molecular features such as COPII- and COPI-coated membranes become apparent. c, As in a, but from a different lamella where no Golgi apparatus is visible. d, As in b. Scale bars, 100 nm.
