Extended Data Fig. 4: Ribosome distribution at and outside of ERES.
From: In situ cryo-ET defines the ultrastructure of ER exit sites in human cells
a. Ribosome coordinates in one example tomogram as defined by template matching are shown as blue spheres. Oversamples points around coated membranes are shown in dark red. Ellipsoid fit around coated membranes to encompass ERES volume is shown in light red. b. Ribosome inclusion zone as defined in ArtiaX by setting a boundary model with alpha = 0.7 (light blue surface). c. As in b, but setting a boundary model with alpha = 1 allows to encompass the full area of the tomogram occupied by cytosol. d. Extrapolated diameters from the volumes of ERES (as from ellipsoid in a, orange), and from the volume of the ribosome exclusion zone (as from the difference between the models in c and b, light blue). e. Distribution of ribosome density inside ERES (orange) and outside the ribosome exclusion zones (light blue). Source numerical data are available in source data.
