Fig. 1: Workflows for competitive isoDTB-ABPP and unbiased analysis of electrophile selectivity. | Nature Chemistry

Fig. 1: Workflows for competitive isoDTB-ABPP and unbiased analysis of electrophile selectivity.

From: Profiling the proteome-wide selectivity of diverse electrophiles

Fig. 1: Workflows for competitive isoDTB-ABPP and unbiased analysis of electrophile selectivity.

a, Workflow for competitive, residue-specific chemoproteomic experiments using the isoDTB-ABPP workflow5. b, Unbiased workflow to comprehensively investigate electrophile reactivity in the proteome using the MSFragger-based FragPipe computational platform31,32. RG, reactive group. D, desthiobiotin; CuAAC, copper-catalyzed azide-alkyne cycloaddition.

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