Fig. 6: A set of 17 electrophilic probes that enables study of nine different amino acid residues and the protein N-terminus.
From: Profiling the proteome-wide selectivity of diverse electrophiles
The heatmap shows the selectivities of a selection of the probes that allow study of diverse residues in the proteome of S. aureus. The colour is scaled by the fraction of all modified sites that were modified at the indicated amino acid. aLabelling was performed using UV-activation at 280–315 nm (MeTet-alkyne, CP-alkyne and MMP-alkyne) or 365 nm (oNBA-alkyne and HMN-alkyne) for 10 min. bData for the indicated probe at 1 mM are shown. cLabelling was performed in degassed lysate under argon. All data are based on technical duplicates.
