Extended Data Fig. 2: Peptides initiated with 2-tRNAfMet or 3-tRNAfMet were translated at levels comparable to those initiated with fMet-tRNAfMet.
From: Chemical and ribosomal synthesis of atropisomeric and macrocyclic peptides with embedded quinolines
Total ion chromatogram of IVT reactions programmed with a duplex DNA template encoding the FLAG-containing polypeptide MGVDYKDDDDK (MGV-flag) in the presence or absence of tRNAfMet acylated with the DNB esters of fMet (fM) or monomers 2 or 3 following anti-FLAG purification as described in the Supplementary Information, Sections III–IV. As previously described by others, we also observe side-products resulting from “drop-off-reinitiation”32,59,113,114,115,116.
