Extended Data Fig. 9: Chemoenzymatic synthesis of a lariat lipopeptide via enzymatic lariat formation followed by Ser/Thr ligation for site-selective acylation.
From: Non-ribosomal peptide cyclase-directed chemoenzymatic synthesis of lariat lipopeptides
a. Synthetic scheme of the lariat lipopeptide cyc-29-SL021. d- and l-configured terminal residues are highlighted with magenta and purple circles, respectively. b. HPLC analysis of reaction products at each synthetic step. Trace A: SurE reaction mixture using 29 as a substrate. Reactions were performed under reaction condition 5, as listed in Supplementary Table 4. Trace B: Reaction mixture after incubation with SL021 in pyridine/acetic acid buffer. The oxazolidine was marked with an asterisk. Trace C: Reaction mixture after TFA treatment. Samples were analyzed under analytical condition 4, as listed in Supplementary Table 5.
