Fig. 2: SurE-mediated chemoenzymatic synthesis of lariat peptides.
From: Non-ribosomal peptide cyclase-directed chemoenzymatic synthesis of lariat lipopeptides
a, Structure of the dipeptide unit used to install the pseudo-N terminus. b, Surugamide B-based branched EG-functionalized substrates 6–11 were synthesized to assess the effect of distance between the pseudo-N terminus and C terminus. c, Conversions of 6–11 using SurE in the absence and presence of 30% DMSO. The conversions to macrocyclization (orange) and EG hydrolysis (blue) products are shown. d, Lariat peptides generated by the reactions of 6–11 with SurE. The Cα atoms of d-aa and l-aa at the substrate termini are highlighted by magenta and purple circles, respectively. The ring sizes are indicated in circles in the structures.
