Fig. 4: Chemoenzymatic synthesis of lariat-shaped lipopeptides by post-cyclization acylation.
From: Non-ribosomal peptide cyclase-directed chemoenzymatic synthesis of lariat lipopeptides
a, The synthetic scheme for the lariat lipopeptide cyc-29-SL021 is shown as an example. After SurE-mediated lariat formation, the enzymatic reaction mixture was directly subjected to STL to introduce the biphenyl group onto the free N-terminal d-Ser that was not used for cyclization. b, The structures of 51 lariat lipopeptides synthesized in this study. The lariat scaffolds (top) and acyl moieties (bottom) are shown. The d- and l-configured terminal residues are highlighted by magenta and purple circles, respectively. The position of the acyl group is shaded in grey. The ring sizes are indicated in circles in the structures.
