Extended Data Fig. 4: Quantitative profiling of arginine reactivity in the human proteome.
From: Global profiling of arginine reactivity and ligandability in the human proteome
a) Waterfall plot of log2(R10:1) values from two biological replicates, representing the probe-modified arginine sites used to quantify arginine reactivity across the proteome. b-d) Validation of labeling for proteins containing hyper-reactive arginine residues (GSHR R81, RIR1 R284, and FSCN1 R217). Recombinant wild-type (WT) proteins and corresponding arginine-to-lysine (R-to-K) mutants were expressed in HEK293T proteomes, labelled with probe 1, and analyzed via in-gel fluorescence. e) Western blots were used to confirm protein expressions for LLPS assays. All data are representative of two biologically independent experiments (n = 2).
