Extended Data Fig. 7: Validation of liganded arginines in cell lysate.
From: Global profiling of arginine reactivity and ligandability in the human proteome
a) Representative MS1 extracted ion chromatograms (XIC) showing decreased probe 1-labelled peptide signal upon competition with CP 1 for KAD1. Average RDMSO/CP values, calculated from biological duplicates, are displayed below the XIC. b) Western blot validation of liganded arginines in HEK293T cell lysates expressing wild-type or arginine-to-alanine mutant KAD1, showing selective blockade of probe 1 labeling by CP 1. c) Structural model of KAD1 (PDB: 2C95) highlighting the liganded arginine residue. d) Bar plots illustrating the effects of CP 1, R81A and R81W mutation on the redox-dependent enzymatic activities of GSHR. Data are shown as mean ± SD from at least three biologically independent replicates.
