Extended Data Fig. 1: Specificity analysis of the probe 1 and 2.
From: Global profiling of arginine reactivity and ligandability in the human proteome
a) Distribution of peptide modifications for probe 1 and 2, considering localized peptide spectrum matches (PSMs) exceeding 80. b) Proposed structures of the probe 1-derived adducts corresponding to the mass shifts of +318.1117 Da and its oxidized form (+334.1066 Da). c) Proposed structures of the probe 2-derived adducts corresponding to the mass shifts of +276.0997 Da and its oxidized product (+292.0961 Da). d) Probe selectivity analysis using an open-search method. e) Selectivity of probe 1 and 2 determined through mass-offset search using the mass shifts in (a). f) Number of modification sites identified by a closed-search using the expected differential masses for each probe.
