Extended Data Fig. 3: Live cell arginine labeling by probe 1.
From: Global profiling of arginine reactivity and ligandability in the human proteome
a) Optimization of probe 1 concentration and incubation time for live-cell labeling. b) Number of arginine-modified sites identified in live cells via closed-search using the expected mass shift of probe 1. c) Cytotoxicity of probe 1 in HeLa cells measured by CellTiter-Glo assay. d) Intracellular concentration of probe 1 in HeLa cells determined by targeted LC–MS/MS. Data are shown as mean ± SD from three biologically independent replicates.
