Extended Data Fig. 2: OED-in-flow pulse program and HPLC time-series.
From: Timed batch inputs unlock substantially higher yields for enzymatic cascades
a, Inflow program comprising a short manual validation segment followed by OED-designed pulses across 10 streams, the species are denoted by the color, the black dashed line represents the total flow rate, the first part of the experiment is manually designed (0-200 min). The in-flow species are: phosphoribosyl pyrophosphate (PRPP), uracil phosphoribosyl transferase (UPRT), phosphoenolpyruvate (PEP), uridine monophosphate kinase (UMPK), adenosine triphosphate (ATP), pyruvate kinase (PK), uracil, adenylate kinase (AK), adenine and adenine phosphoribosyl transferase (APRT). b, Representative time-series for adenine, ATP, uracil, UMP, UTP, and ADP used to train and select mechanistic ODE models. c, Demonstrates that the model fits to complex time-series data.
