Fig. 4: The number of observed homoplasious amino acids relative to the number expected between pairs of vertebrates, plotted against their genetic distances.
Homoplasious amino acids between all pairs of vertebrates that do not share a most recent ancestral node in the fixed tree topology were identified. The number of homoplasious amino acids was divided by the number expected under the neutral JTT-Fsite model to yield a ratio (R; y axis). The x axis shows genetic distance calculated as the number of amino acid substitutions per site between the younger ends of the two branches considered. The black dashed line indicates the linear regression with R 2 = 0.2351. Yellow diamonds show comparisons between thylacine and other vertebrates. Red circles indicate comparisons between the thylacine and key eutherian nodes (Canis, Vulpes, Caninae, Caniformia and Carnivora).
