Fig. 6: Phylogenetic reconstruction of toll-like receptors TLR 1 and TLR 5.
From: Clonal polymorphism and high heterozygosity in the celibate genome of the Amazon molly

Neighbour-joining trees (unrooted) are shown based on the amino acid sequences of the largest open reading frame of innate immune genes TLR 1 (802aa) and TLR 5 (759aa). Only bootstrap support values >50% are shown (1,000 randomizations). Species are marked by coloured squares after the sample names. The colour used for the names corresponds to the mt haplotype (see Supplementary Fig. 2; black, individual haplotype). For both TLRs only very few individuals from each of the parental species showed two different alleles, whereas each P. formosa individual always has two clearly divergent alleles (L indicates P. latipinna origin, M indicates P. mexicana origin), reflecting the hybrid origin of the species.