Extended Data Fig. 1: The high idiosyncrasy indices (Iid) observed are not due to phenotype measurement errors or the use of standard deviation (SD) instead of range of mutational effects.
From: Idiosyncratic epistasis creates universals in mutational effects and evolutionary trajectories
a, SD-based Iid of the yeast tRNA fitness landscape is insensitive to the number of experimental replicates used in the fitness estimation. Boxplots show the distribution of Iid values of 828 single mutations in the tRNA landscape, calculated based on different numbers of replicates. The lower and upper edges of a box represent the first (qu1) and third (qu3) quartiles, respectively, the horizontal line inside the box indicates the median (md), the whiskers extend to the most extreme values inside inner fences, md ± 1.5(qu3 − qu1), and the grey dots represent values outside the inner fences (outliers). Violet dots show mean Iid of all mutations calculated based on respective numbers of replicates. b, Range-based Iid for various phenotype landscapes. Error bars show standard errors. Detailed information of each landscape is provided in Supplementary Table 1. Shown in red is the fraction of mutations exhibiting sign epistasis in each phenotype landscape.
