Extended Data Fig. 1: GABABR modulators inhibit planulae-to-polyp transformation.
Confocal sections at 5 dpf (a-d) and 8 dpf (i-l) labeled with antibodies against phalloidin (green) and DAPI (blue). DIC images of the aboral/apical tuft at 5 dpf (e–h) and 8 dpf (m-p). Control planulae (a,i) and primary polyps (i, m) are shown, as are planulae treated with GABA (b, f, j, n), baclofen (c, g, k, o) or CGP-7930 (d, h, I, p). While at 5 dpf all planulae possessed an apical tuft (numbers are not shown), in 8 dpf control primary polyps after metamorphosis (m), the apical tuft was lost, whereas treated 8 dpf planulae (n–p) still maintained it. The fraction of similar phenotypes from the total number of analyzed samples is given in the lower right-hand corner. Scale bars, 50 µm.
