Extended Data Fig. 6: Platynereis c-opsin1Δ8/Δ8 allele exhibit lowered locomotor activity under longday, including UVA during LD and DD. | Nature Ecology & Evolution

Extended Data Fig. 6: Platynereis c-opsin1Δ8/Δ8 allele exhibit lowered locomotor activity under longday, including UVA during LD and DD.

From: Seasonal variation in UVA light drives hormonal and behavioural changes in a marine annelid via a ciliary opsin

Extended Data Fig. 6

(a-c) Double plotted average actogram plot of c-opsin1+/+ (a: n = 17), c-opsin1Δ8/+ (b: n = 12) and c-opsin1Δ8/Δ8 worms (c: n = 20) under longday, including strong UVA under Light-Dark (LD, days 1-5) and Dark-Dark condition (DD, days 6-10, grey shaded). (d) Significant difference in rhythmicity power (PN) exist for c-opsin1Δ8/+ vs c-opsin1Δ8/Δ8 and c-opsin1+/+ vs c-opsin1Δ8/Δ8, but not for c-opsin1+/+ vs c-opsin1Δ8/+ (Mann-Whitney-Wilcoxon test). (e) Percentage of rhythmicity calculated for all genotypes under LD condition (c-opsin1+/+: 100%R, c-opsin1Δ8/+: 100%R and c-opsin1Δ8/Δ8: 90%R+10%WR). (f) c-opsin1Δ8/Δ8 worms showed significant decrease in nocturnal locomotor activity compared to c-opsin1+/+ and c-opsin1Δ8/+ (One-way ANOVA with Sidak’s multiple comparison test). (g) Under DD free-running conditions, significant differences in rhythmicity power (PN) exist for c-opsin1+/+ vs c-opsin1Δ8/Δ8 and c-opsin1+/+ vs c-opsin1Δ8/+, but no difference for c-opsin1Δ8/+ vs c-opsin1Δ8/Δ8 (Mann-Whitney-Wilcoxon test). (h) Percentage of rhythmicity calculated for all genotypes under DD condition (c-opsin1+/+: 76.48%R+17.64%WR+5.88%AR, c-opsin1Δ8/+: 33.33%R+41.67%WR+25%AR and c-opsin1Δ8/Δ8: 30%R+30%WR+40%AR). (i) c-opsin1Δ8/Δ8 worms recorded under DD condition showed significant decrease in nocturnal locomotor activity compared to c-opsin1+/+ and c-opsin1Δ8/+ (One-way ANOVA with Sidak’s multiple comparison test). *p<0.05, ** p<0.01, *** p<0.001. For individual actograms see Supplementary Fig. 2.

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