Extended Data Fig. 8: Locomotion under long day (LD 16:8) and intermediate photoperiod (LD 12:12) with full and filter-reduced UVA.

Locomotor behaviour of Platynereis c-opsin1Δ8/Δ8 mutant and its corresponding wt siblings. (a,b) Double plotted average actograms of c-opsin1+/+ worms under long day Nelis white light with intense UVA (+UVA) (a: n = 12) and with filter-reduced UVA (-UVA) (b: n = 10). (c) c-opsin1+/+ worms under –UVA conditions showed a significantly decrease locomotor activity compared to worms under +UVA conditions and (d) significant decrease in power (PN) and rhythmicity. (e,f) Double plotted average actograms of c-opsin1Δ8/Δ8 worms under LD16:8 +UVA (e, n = 11) and -UVA (f, n = 10). (g,h) c-opsin1Δ8/Δ8 worms recorded in (e,f) showed no difference in locomotor activity level (g) and rhythmicity (h). (i,j) Double plotted average actograms of c-opsin1+/+ worms under LD 12:12 +UVA (i: n = 9) and -UVA (j: n = 7). (k,l) c-opsin1+/+ worms recorded in (i,j) with a difference in locomotor activity close to statistical significance (k), and no difference in rhythmicity (l). (m,n) Double plotted average actograms of c-opsin1Δ8/Δ8 worms under LD 12:12 +UVA (m: n = 10) and -UVA (n: n = 9). (o,p) c-opsin1Δ8/Δ8 worms recorded in (m,n) showed no difference trend in locomotor activity level (o) and rhythmicity (p). For all statistical comparisons and p values: Supplementary Fig. 7. Statistics: locomotor activity: One-way ANOVA, Sidak’s multiple comparison test, period, power and rhythmicity: Individual worm rhythmicity and power were determined via Lomb-Scargle periodograms using ActogramJ. The averages of multiple worms were tested by Mann-Whitney-Wilcoxon test. *p<0.05, ** p<0.01, *** p<0.001. Locomotor data of individual worms: Supplementary Figs. 8, 9.