Extended Data Fig. 1: Detection of putative spontaneous cell fusions.
From: Spontaneous cell fusions as a mechanism of parasexual recombination in tumour cell populations
a. Live-cell fluorescence microscopy images of 2D co-cultures between differentially labelled (nuclear GFP and mCherry) cell lines. Arrowheads indicate cells that express both labels. b. Live-cell fluorescence microscopy images of 3D Matrigel co-cultures between MCF10DCIS breast carcinoma cells and a primary breast CAF isolate labelled with cytoplasmic GFP and dsRED, respectively. Arrowheads indicate cells that express both labels. c,d Time-lapse live fluorescence microscopy images of mCherry+ SUM159PT cells co-cultured with GFP+ MDA-MB-231 cells (c) and CAFs (d). The labels indicate time after plating. Black arrowheads show fusion parents, and white arrowheads show double-positive hybrid cells and their progeny. e. Quantification of flow cytometry detection of double-positive events in the co-cultures between GFP+ CAFs and indicated breast cancer cell lines labelled with mCherry. Error bars represent SD, each dot represents an independent biological replicate. *and ** denote p values below 0.05 and 0.01 for two-tailed unpaired t-test, respectively.
