Extended Data Fig. 4: DNA content analysis of hybrid cells.
From: Spontaneous cell fusions as a mechanism of parasexual recombination in tumour cell populations
a. FACS analysis of DNA content of the indicated parental cell lines and their hybrids. P1-12 indicate passage number of the mixed hybrid populations; for hybrid subclones the number indicates passage of the mixed culture, used for isolation of single cell subclones. Black contours indicate DNA content profiles for parental cell lines and their G1 and G2 peaks are used as reference points, filled histograms indicate DNA content profiles of the hybrids. “Unstable genomes” and “stable genomes” refer to hybrids that, respectively, did and did not show evidence of reduced DNA content between early and extended passages. Hybrids with stable genomes demonstrate G1 peak closer to G2 peak of parents and no observed difference in DNA content between passages and single cell clones. Inset indicates axes and shows components of the merged plots. b. FACS analysis of retention of GFP and mCherry fluorescence in hybrid cells, recovered from metastasis-bearing lungs of the indicated animals depicted in Fig. 2g.
