Extended Data Fig. 5: Plots used to determine gating parameters for flow cytometry from one representative sample. | Nature Ecology & Evolution

Extended Data Fig. 5: Plots used to determine gating parameters for flow cytometry from one representative sample.

From: Experimental manipulation of microbiota reduces host thermal tolerance and fitness under heat stress in a vertebrate ectotherm

Extended Data Fig. 5

a, A plot of fluorescein isothiocyanate (FITC) vs. cell counts was used to distinguish cells stained with SYBR Green dye from all other cells b, A plot of forward scatter (FSC) vs. side scatter (SSC) was used to distinguish populations of counting beads from all other events. To establish initial gates for counting beads, blank samples spiked only with beads were used. On both plots, red rectangles represent the gates and surround the events counted. Percentages indicate the proportion of events within the gate out of total events. R1 = stained bacterial cells and R2 = counting beads.

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