Extended Data Fig. 4: Experiments with K-12 MG1655 confirmed the generality of the transposition to plasmid mechanism across E. coli strains.
From: Intra- and interpopulation transposition of mobile genetic elements driven by antibiotic selection
(a) qPCR and (b) DNA electrophoresis demonstrate that the fraction of cells with the transposon on the plasmid, Sp, increases as antibiotic concentrations increase (data shown as mean ± S.D., n = 3). The MG1655 strain is used as the genetic background, and the same genetic constructs that were used in DH5α for previous experiments (See Fig. 2), were inserted into the homologous genomic region (strain S127). The top arrow in the panel (b) indicates the plasmid with the transposon insertion, and the bottom arrow indicates the empty plasmid. Two independent repeated experiments were performed for the DNA electrophoresis experiments (b).
