Extended Data Fig. 6: Experiments with K-12 MG1655 demonstrated the generality of the transposition to plasmid mechanism, across E. coli strains, and under the control of different promoters.
From: Intra- and interpopulation transposition of mobile genetic elements driven by antibiotic selection
qPCR showed transposon copy numbers increased with increasing antibiotic concentrations for synthetic transposons with different basal expression levels of the tetA resistance gene (mean ± S.D., n = 3). qPCR was performed on MG1655 strains containing the same high-copy-number plasmid, but with chromosomal-based transposons under the control of different promoters: a weak promoter in strain S128 (a) or a medium promoter in strain S129 (b).
