Extended Data Fig. 8: The serial inoculation protocol in experiments with native transposons.
From: Intra- and interpopulation transposition of mobile genetic elements driven by antibiotic selection
As native-derived transposons may not be as active as the synthetic miniTn5-derived transposons, we used serial inoculation experiments to select for higher transposon copy numbers. Cultures were inoculated into deep well plates with increasing tetracycline concentrations over time. The protocol enriched cells with plasmid-borne transposons, which were hard to detect using the protocol designed for miniTn5-transposons.
